Spinal cord injury-induced neurogenic detrusor overactivity

Model’s advantageS

The most commonly utilised and highly informative model of a central lesion with respect to lower urinary tract function
used for the evaluation of drugs targeting neurogenic detrusor overactivity (NDO) but also overactive bladder whatever its etiology
useful to investigate the effect of mechanisms known to act on C-fiber afferents
useful to investigate an effect on the external urethral sphincter activity

SPECIES

rat

Pathophysiological featureS

Mimics the voiding patterns of patients with neurogenic detrusor overactivity due to spinal cord injury
Display neurogenic detrusor overactivity characterized by non-voiding contractions during the filling phase with increased maximal micturition pressure and increased micturition duration.
Reduced voiding efficiency and large residual urine volume associated with detrusor-sphincter dyssynergia
Bladder hypertrophy
Increase in bladder afferent nerve activity, in particular through C-fibers
BBB score impairment altered locomotor activity (reduced BBB score)

Figure 1: Representative cystometrograms in conscious 3 weeks SCI and sham rats. Arrows indicate the voiding contractions. Neurogenic detrusor overactivity occured in the SCI rat but not in sham rats. (Pelvipharm, internal data).

Summarized methodology

A T7-T8 laminectomy is performed and the spinal cord is cut between T7 and T8 vertebrae. A sterile gelform sponge is placed between the cut ends of the spinal cord.

Neurogenic detrusor overactivity progressively develops over time until 3-4 weeks where it is stabilized.

Figure 2: Complete transection between vertebral T7-T8.

Related Pelvipharm bibliography

Broqueres-You, D. et al. Basic & Clinical Pharmacology & Toxicology, 107 (Suppl. 1), 192 (WorldPharma 2010)

Behr-Roussel, D. et al. J Urol (2010) : 183(4),supl1:e391 (AUA, 2010)

Broqueres-You, D. et al. J Urol (2010) : 183(4),supl1:e76-e77 (AUA, 2010)

Behr-Roussel, D. et al. Eur Urol Suppl (2010) : 9(2):73 (EAU, 2010)

Broqueres-You, D. et al. Eur Urol Suppl (2010) : 9(2):112 (EAU, 2010)

Broqueres-You, D. et al. Neurourol Urodyn (2009) : 28(7):695 (ICS, 2009)

Broqueres-You, D. et al. J Urol Abstract (AUA, 2008) : 179(4) : 348-349

Links to applicable Experimental skills

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