/* */ PELVIPHARM - Acetic acid evoked bladder irritation
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Acetic acid evoked bladder irritation

Model’s advantages

  • Evokes bladder irritant responses, in particular through C-fibers
  • Mimics the increased sensory activity, which occurs in overactive bladder and urge incontinence
  • Useful for quick investigation of the effect of drugs known to act on either C-fiber afferents or bladder smooth muscle

Species

rat, guinea pig

Pathophysiological features

  • Cystometrogram displays:
    Increase in micturition pressure,
    Increase in micturition frequency 
    Decrease in pressure threshold to evoke a bladder contraction
    Decrease in bladder capacity and voided volume 
    Reduction in bladder compliance,
  • Increase C-Fos positive cells in the spinal cord (L6 level)
Figure 1: Representative cystometrograms showing the effect of acetic acid-induced bladder hyperactivity in anesthetized female rat. Arrows indicate the voiding contractions. (Pelvipharm, internal data).
Figure 1: Representative cystometrograms showing the effect of acetic acid-induced bladder hyperactivity in anesthetized female rat. Arrows indicate the voiding contractions. (Pelvipharm, internal data).
  • Guinea pig are usually used for acetic acid-induced bladder hyperactivity experiments because they display very stable and reproducible micturition cycles allowing the obtention of very reliable experiments 
Figure 2: Representative cystometrograms showing the effect of acetic acid-induced bladder hyperactivity in anesthetized female guinea pig. Arrows indicate the voiding contractions. (Pelvipharm, internal data).
Figure 2: Representative cystometrograms showing the effect of acetic acid-induced bladder hyperactivity in anesthetized female guinea pig. Arrows indicate the voiding contractions. (Pelvipharm, internal data).

SUMMARIZED METHODOLOGY

The bladder is perfused with continuous acetic acid (0.1% for guinea pigs, 0.5% for rats) at a rate of 50 µl/min (rat) or 300 µl/min (guinea pig) while intravesical and blood pressure are monitored concomitantly.

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