Lipopolysaccharide-induced bladder inflammation
Model’s advantages
- The endotoxin lipopolysaccharide is extensively used in the development of many organ specific inflammatory disease models.
- The intravesical LPS infusion induces similar pathophysiology features observed in patients with interstitial cystitis
- LPS damages the urothelium and produces a marked bladder overactivity involving the stimulation of C-fiber afferent
- Useful to evaluate the effect of a drug in chronic inflammatory conditions
SPECIES
Rat
Summarized methodology
Bladder inflammation is induced by intravesical infusion of protamine sulphate (PS, 10 mg/ml) during 1 hour in order to alter mucosal permeability followed by intravesical infusion of lipopolysaccharide (LPS, 1 mg/ml) during another 1 hour. Bladder inflammation is transient, peaking 4 hours after PS/LPS instillation and resolving by 72 hours. Urodynamic evaluation can then be performed at the inflammatory peak.
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Non disclosable information for confidentiality reasons
Links to applicable Experimental skills
- Administration routes / Regimen
- Confocal Microscopy
- Immunohistology
- Metabolic cages (diuresis, renal function, spontaneous micturition)
- Morphology
- Morphometry
- Organ bath with animal tissues (In Vitro studies)
- Oxidative fluorescence
- Plasma / urine / tissue collection
- Protein expression and activity
- Spectrophotometric assays
- Telemetry
- Urodynamic evaluation (anesthetized)
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