In vitro organ baths functional experiments with animal tissues
Animal tissues
- In vitro functional investigation of animal tissue function in normal and pathophysiological conditions.
- Unrestricted amount of tissue.
- Evaluation of the ability of drugs at modulating different smooth muscle tone can be performed on contractile or relaxant response elicited by pharmacological agents or by electrical field stimulation (EFS).
- Evaluation of mRNA by RT-PCR or protein expression, by immunohistochemistry (IHC) or western-blot (WB), in parallel of organ bath studies.
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CRC = concentration-response curve |
NB: Pelvipham will gladly study the feasibility of performing additional in vitro assays on other species to meet its client’s needs.
Endpoints
- Evaluation of the capacity of a drug to inhibit detrusor smooth muscle contractile activity.
- Determination of potency (EC50) and efficiency (Emax) of a drug.
- Determination of the affinity (pA2) of a drug for a human bladder receptor.
Related Pelvipharm bibliography
Oudot A. et al.
Physiol Res (2009) : 58(4):499-509
Behr-Roussel D. et al.
Eur Urol (2008) : 53(6):1272-1281
Oger S. et al.
J Urol (2007) : 177(4): # 257 (AUA, 2007)
Oger S. et al.
Eur Urol (2007) : 6(2):272 (EAU, 2007)
Behr-Roussel D. et al.
Eur. Urol. (2005) : 47(1):87-91
Behr-Roussel D. et al.
Am. J. Physiol. (2005) : 288(1):R276-283
Behr-Roussel D., et al.
Am. J. Physiol. (2003) : 284(3):R682-688
Physiol Res (2009) : 58(4):499-509
Behr-Roussel D. et al.
Eur Urol (2008) : 53(6):1272-1281
Oger S. et al.
J Urol (2007) : 177(4): # 257 (AUA, 2007)
Oger S. et al.
Eur Urol (2007) : 6(2):272 (EAU, 2007)
Behr-Roussel D. et al.
Eur. Urol. (2005) : 47(1):87-91
Behr-Roussel D. et al.
Am. J. Physiol. (2005) : 288(1):R276-283
Behr-Roussel D., et al.
Am. J. Physiol. (2003) : 284(3):R682-688
Links to applicable Targeted disorders / Pathophysiological models
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