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In vitro organ baths functional experiments on human tissue

Objectives

  • Direct investigation of human tissue physiology and pharmacology, thus very relevant in terms of "transposition" to human pathologies.
  • Evaluation of ex vivo contraction and relaxation processes of various smooth muscles.
  • Unique opportunity to evaluate the effect of a drug and its mechanism of action on human physiological functions at the preclinical stage of development.
  • In vitro evaluation of the effect of drugs at modulating smooth muscle tone in normal or pathological conditions.
  • Determination and comparison of potency (EC50), efficiency (Emax) and tissue selectivity of drugs by performing concentration response curves.
  • Determination of drug affinity (pA2) for a specific receptor and a specific tissue.

Human tissue available

  • Bladder from control patients (with no known bladder dysfunction according to their medical chart).
  • Bladder from neurogenic patients
  • Prostate from control patients
  • Prostate from patients with benign prostatic hyperplasia
  • Corpus cavernosum
  • Seminal vesicles

Other tissues can be obtained upon specific request

Source of human tissues sample

  • Pelvipharm collaborates with public and private hospitals located in Paris and other regions all over France.
  • The collection and use of tissue is carried out in accordance with the Research Plan, all relevant laws, regulations and codes of practice (including obtaining informed consent of patients in writing and determination of hepatitis and HIV serologies).
  • Human tissues are transported to Pelvipharm facilities via a specialized carrier ensuring security and adapted condition of storage

Human tissue preparation

  • Sections are excised from the sample of each donor for each experiment.
  • Tissue strips are mounted isometrically to force transducers in organ bath filled with Krebs-HEPES buffer (37°C; 95% O2 and 5% CO2).
  • Following amplification, the tension changes are digitalized via a Mac Lab TM/8 using Chart TM 5 software (AD Instruments Ltd, Australia).
  • Appropriate pharmacological agents and/or electrical field stimulation are then applied on the tissue strips after adequate priming of the tissue.
Figure 1: Overview of an organ-bath set-up
Figure 1: Overview of an organ-bath set-up.
Related Pelvipharm bibliography
Oger, S. et al. BJP (2010) : Accepted

Oger, S. et al. Eur Urol (2010) : 57(4):699-707

Oger, S. et al. J Sex Med (2009) : 6(3) : 836-847

Oger, S. et al. J Urol (2009) : 181(4):568 (AUA, 2009)

Behr-Roussel, D. et al. J Urol (2009) : 181(4):568 (AUA, 2009)

Giuliano, F. et al. J Urol (2009) : 181(4) : 693 (AUA, 2009)

Behr-Roussel, D. et al. Eur Urol Supl (2009) : 8(4):190 (EAU, 2009)

Oger, S. et al. J Sex Med (2008) : 5(4) : 935-945

Oger, S. et al. J Urol (2008) : 179(4) : 352 (AUA, 2008)

Oger, S. et al. J Urol (2008) : 179(4):128 (AUA, 2008)

Behr-Roussel, D. et al. J Urol (2008) : 179(4):541 (AUA, 2008)

Oger, S. et al. Eur Urol Supl (2008) : 7(3) : 183 (EAU, 2008)

Oger, S. et al. Eur Urol Supl (2008) : 7(3):263 (EAU, 2008)

Behr-Roussel, D. et al. Eur Urol Supl (2008) : 7(3):263 (EAU, 2008)

Oger, S. et al. Eur Urol (2007) : 51(3) : 772-781

Oger, S. et al. Neurourol Urodyn (2007) : 26(5) (ICS, 2007)

Darblade, B. et al. Urology (2006) : 68(2) : 442-448

Darblade, B. et al. World J Urol (2005) : 23(2) :147-151
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