/* */ PELVIPHARM - Neuro-anatomical tracing techniques
Receive our news


Neuro-anatomical tracing techniques


Several anterograde and retrograde neuronal tracers have been developed for respectively evidencing afferent and efferent neural pathways. These tools have largely contributed to the understanding of neuroanatomical connections between different central or peripheral organs. Recently, a new class of neuronal tract tracers consisting of modified virus, has been efficaciously developed and used (Enquist, 2005).

Summarized methodology

The general procedure consists in injecting the neuronal tracers in the organs or regions of interest. When these injections are performed in the brain, animals are placed in a stereotaxic frame and brain canulae are used for the injection of the tracers. After a post-survival period depending on the properties of the neuronal tract tracer used, animals are sacrificed and the organs of interest are collected and sliced. Then, slices are directly examined using a microscope under epifluorescence if fluorescent tracers are used (cf. confocal microscopy), or after immunocytochemical labelling if the tracers have to be detected.


  • Qualitative analysis of the localisation of the neuronal tract tracers in organs or regions of interest
  • Number and percentage of single, double or triple-labeled cells

Related Pelvipharm bibliography
Gelez, H et al.
J Sex Med (2010) - Accepted
TEL +331 704 293 39
FAX + 331 704 295 10