Streptozotocin-induced diabetic rats-mice (STZ)_Type I diabetes
Model advantages
The rat / mouse model of diabetes mellitus induced by streptozotocin injections is one of the most widely used model of type I diabetes and mimics human pathological situation of decreased insulin production (untreated type I diabetes) or decrease tissue responsiveness to insulin.
Pathophysiological features
Metabolic features:
- weight loss
- hyperglycemia
- Streptozotocin–induced pancreatic beta cells (insulin-producing cells) destruction
- Autonomic neuropathy
Cardiovascular features:
- Vascular endothelial dysfunction (aorta) (figure 1)
- Acceleration of atherosclerosis progression in ApoE knockout mice
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| Figure 1: Comparison of endothelium-dependent relaxations in control and streptozotocin-induced diabetic rats obtained in in vitro experiments performed in aortic rings. (Pelvipharm, internal data). |
Erectile function features:
- Impairement of endothelial and neurogenic relaxations of corpora cavernosa (figure 2)
- Penile autonomic neuropathy
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| Figure 2: Comparison of endothelium-dependent and EFS-induced relaxations in control and streptozotocin-induced diabetic rats obtained in in vitro experiments performed in cavernosal strips (Pelvipharm internal data). |
Summarized methodology
In rats or mice, streptozotocin intra-peritoneal injection(s) induces diabetes which is confirmed by determining blood glucose levels
Related Pelvipharm bibliography
Non disclosable information for confidentiality reasons
Links to applicable Experimental skills
- Administration routes / Regimen
- Confocal Microscopy
- Immunohistology
- Metabolic cages (diuresis, renal function, spontaneous micturition)
- Morphology
- Morphometry
- Non invasive blood pressure monitoring (tail cuff)
- Organ bath with animal tissues (In Vitro studies)
- Oxidative fluorescence
- Plasma / urine / tissue collection
- Protein expression and activity
- Spectrophotometric assays
- Telemetry
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