/* */ PELVIPHARM - Protein expression Immunohistochemistry - Immunofluorescence
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Protein expression Immunohistochemistry - Immunofluorescence

Objectives

Immunohistochemistry allows evidencing the presence a protein within a tissue section but also its localization within the tissue. This method may help to better characterize the cellular component targeted by an active compound and/or its mechanism of action.

Summarized methodology

  • Immunohistochemistry can be performed on paraffin-embedded tissue sections or cryosections.
  • Immunostaining can be performed with the use of an enzyme-coupled (HRP or AP) or with a biotinylated secondary antibody when signal amplification (biotin-streptavidin-HRP complex) is needed
  • Immunological reaction is detected using either a colorimetric enzymatic reaction or a fluorescent dye

Endpoints

  • Expression and localization of protein of interest
  • semi-quantitative evaluation of the level of expression of a protein of interest (figure 1)
C_Imm_His_AO_1_1.jpg Figure 1: Representative images of semi-quantitative scoring of immunostaining (PCNA) in rat prostate sections depending on intensity of the staining (pelvipharm internal data).
Figure 1: Representative images of semi-quantitative scoring of immunostaining (PCNA) in rat prostate sections depending on intensity of the staining (pelvipharm internal data).
Related Pelvipharm bibliography
Gelez, H et al.
J Sex Med (2010) : 7 (suppl 4):151 (ISSM 2010)
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