Protein expression – Immunohistochemistry - Immunofluorescence
Immunohistochemistry allows evidencing the presence a protein within a tissue section but also its localization within the tissue. This method may help to better characterize the cellular component targeted by an active compound and/or its mechanism of action.
- Immunohistochemistry can be performed on paraffin-embedded tissue sections or cryosections.
- Immunostaining can be performed with the use of an enzyme-coupled (HRP or AP) or with a biotinylated secondary antibody when signal amplification (biotin-streptavidin-HRP complex) is needed
- Immunological reaction is detected using either a colorimetric enzymatic reaction or a fluorescent dye
- Expression and localization of protein of interest
- semi-quantitative evaluation of the level of expression of a protein of interest (figure 1)
|Figure 1: Representative images of semi-quantitative scoring of immunostaining (PCNA) in rat prostate sections depending on intensity of the staining (pelvipharm internal data).|
J Sex Med (2010) : 7 (suppl 4):151 (ISSM 2010)
Links to applicable Targeted disorders / Pathophysiological models