Confocal laser scanning microscopy is a technique developed for obtaining high-resolution optical images. This technique is particularly useful to examine double or triple immunofluorescent labelling, developed for example to examine the distribution and localization of different molecules (receptors, peptides, enzymes, etc…) on same tissues.
Fluorescent immunocytochemistry using antibodies against molecules of interest is performed on slices of tissue. Then, high-resolution optical images are acquired using confocal laser scanning microscopy which allows the analysis and quantification of the labelling.
- localisation of molecules of interest
- number and percentage of double or triple labeled positive cells
J Sex Med (2010) : 7(6):2056-2067
Gelez, H. et al.
(ESSM/ISSM 2008) : Oral presentation
Links to applicable Targeted disorders / Pathophysiological models