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In addition to organ weighing, and morphological examination, several histomorphometric measurements can be performed to quantitatively evaluate the remodeling of a tissue.

Summarized methodology

Following tissue staining, images are captured using a light microscope (Eclipse E800, Nikon, France – Figure 1) connected to a video camera (Digital camera DXM1200, Nikon, France), and histomorphometric measurements are performed by computerized image analysis system (NIS-elements software®).

Figure 1: Overview of the microscope set-up.

Figure 1: Overview of the microscope set-up.


Different parameters can be evaluated according to the type of tissue to be examined

Related Pelvipharm bibliography
Behr-Roussel, D. et al.
J Sex Med (2006) : 3(4):596-603

Behr-Roussel, D. et al.
Atherosclerosis (2002) : 162:355-362
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