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Spectrophotometric assays


Spectrophotometric assays allows the determination of the concentration of a substance of interest in various biological samples (urine, blood, plasma, tissue) and helps to better understand the mechanism of action of an active compound.

Summarized methodology

A wide range of assays have been validated based on direct colorimetric reactions or immuno-enzymatic detection (EIA / ELISA).

Pelvipharm is used to collect various sample types (urine, blood, plasma, tissue), while taking special care to adopt an appropriate storage of samples and to perform pre-analytical steps (extraction when needed) before any biochemical spectrophotometric assay.

Absorbance reading is performed using a Molecular Devices microplate reader (Spectramax 190) associated to SoftMax® Pro microplate analysis software.


A wide range of biochemical spectrophotometric assays have been validated at Pelvipharm, as follows :

NB: Pelvipharm will gladly study the feasibility of performing additional spectrophotometric assays to meet its client’s needs.

Related Pelvipharm bibliography
Oudot, A. et al.
J Sex Med (2010) : 7(1)p1:79-88

Oudot, A. et al.
Physiol Res (2009) : 58(4):499-509

Behr-Roussel, D. et al.
Eur Urol (2008) : 53(6):1272-1281

Behr-Roussel, D. et al.
Am J Hypertens (2008):21(11):1258-1263

Behr-Roussel, D. et al.
Am J Physiol – Regul (2005) : 288:R276-R283

Behr-Roussel, D. et al.
Atherosclerosis (2002) : 162:355-362
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