Acetic acid evoked bladder irritation
Model’s advantages
- Evokes bladder irritant responses, in particular through C-fibers
- Mimics the increased sensory activity, which occurs in overactive bladder and urge incontinence
- Useful for quick investigation of the effect of drugs known to act on either C-fiber afferents or bladder smooth muscle
Species
rat, guinea pig
Pathophysiological features
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Cystometrogram displays:Increase in micturition pressure,Increase in micturition frequencyDecrease in pressure threshold to evoke a bladder contractionDecrease in bladder capacity and voided volumeReduction in bladder compliance,
- Increase C-Fos positive cells in the spinal cord (L6 level)
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| Figure 1: Representative cystometrograms showing the effect of acetic acid-induced bladder hyperactivity in anesthetized female rat. Arrows indicate the voiding contractions. (Pelvipharm, internal data). |
- Guinea pig are usually used for acetic acid-induced bladder hyperactivity experiments because they display very stable and reproducible micturition cycles allowing the obtention of very reliable experiments
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| Figure 2: Representative cystometrograms showing the effect of acetic acid-induced bladder hyperactivity in anesthetized female guinea pig. Arrows indicate the voiding contractions. (Pelvipharm, internal data). |
SUMMARIZED METHODOLOGY
The bladder is perfused with continuous acetic acid (0.1% for guinea pigs, 0.5% for rats) at a rate of 50 µl/min (rat) or 300 µl/min (guinea pig) while intravesical and blood pressure are monitored concomitantly.
Related Pelvipharm bibliography
Non disclosable information for confidentiality reasons
Links to applicable Experimental skills
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