Lipopolysaccharide-induced bladder inflammation
- The endotoxin lipopolysaccharide is extensively used in the development of many organ specific inflammatory disease models.
- The intravesical LPS infusion induces similar pathophysiology features observed in patients with interstitial cystitis
- LPS damages the urothelium and produces a marked bladder overactivity involving the stimulation of C-fiber afferent
- Useful to evaluate the effect of a drug in chronic inflammatory conditions
Bladder inflammation is induced by intravesical infusion of protamine sulphate (PS, 10 mg/ml) during 1 hour in order to alter mucosal permeability followed by intravesical infusion of lipopolysaccharide (LPS, 1 mg/ml) during another 1 hour. Bladder inflammation is transient, peaking 4 hours after PS/LPS instillation and resolving by 72 hours. Urodynamic evaluation can then be performed at the inflammatory peak.
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