/* */ PELVIPHARM - Cyclophosphamide-induced bladder irritation
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Cyclophosphamide-induced bladder irritation

Modelís advantages

  • Cyclophosphamide is a pro drug chemotherapeutic agent which ultimately leads to the formation of acrolein.
  • Acrolein damages the bladder urothelium and can lead to hemorrhagic cystitis. Moreover, it  produces a marked bladder overactivity mediated through the stimulation of C-fiber afferent.
  • Useful to evaluate the effect of a drug on bladder inflammatory processes such as interstitial cystitis.

Species

Rat

pHYSIOLOGICAL FEATURES

  • Abnormal bladder function; cystometrogram displays a decrease in intercontraction interval and a decrease in bladder capacity.
  • Hematuria.
  • Bladder wall edema.
  • Increase in bladder weight.
  • inflammatory cell (polymorphonuclear neutrophils) infiltration in the bladder lamina propria.
  • Increase C-Fos positive cells in the spinal cord (L6 level).
Figure 1: Representative pictures illustrating the inflammatory effect of cyclophosphamide on bladders from cyclophosphamide (CYP) treated rats. (From Giuliano.f et al. 2006).
Figure 1: Representative pictures illustrating the inflammatory effect of cyclophosphamide on bladders from cyclophosphamide (CYP) treated rats. (From Giuliano.f et al. 2006).


Figure 2 : Representative cystometrograms in anesthetized rats showing the effect of cyclophosphamide pre-treatment. (PVP, internal data).
Figure 2 : Representative cystometrograms in anesthetized rats showing the effect of cyclophosphamide pre-treatment. (PVP, internal data).

Summarized methodology

Rats are injected with vehicle or cyclophosphamide intraperitoneally (i.p) at the dosing of 100 mg/kg 48h before testing. Thereafter, urodynamic evaluation can then be performed (cf. Links to applicable experimental skills)

Related Pelvipharm bibliography
Giuliano, F. et al.
Brit J Urol (2006) : 97(2):386-392
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