L-NAME induced hypertension in rats - mice
Model advantages
This experimental model easily creates generalized nitric oxide (NO) deficiency that if prolonged, is responsible for a progressive increase in arterial pressure associated cardiovascular remodeling.
Pathophysiological features
Cardiovascular features :
- progressive increase of mean arterial pressure
- cardiac and vascular remodeling if treatment period is sufficiently prolonged
Erectile function features :
- Dramatic impairment of erectile responses to electrical stimulation of the cavernous nerve after 4 weeks of L-NAME administration (10 or 50 mg/kg/d in drinking water) in anesthetized rats (figure 1).
- Dose dependent decrease erectile responses to electrical stimulation of the cavernous nerve following acute L-NAME intravenous injection (1 or 3 mg/kg) in anesthetized rats (figure 1).
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| Figure 1: Effects of acute L-NAME (1 or 3 mg/kg i.v.) and chronic L-NAME (10 or 50 mg/kg/d p.o. for 4 weeks) on intracavernosal pressure (ICP) after ES CN in anesthetized rats (Pelvipharm internal data). |
Pathophysiological
- Chronic L-NAME is administered orally in drinking water
- Acute L-NAME is administered intravenously at the time of experiment
Related Pelvipharm bibliography
Non disclosable information for confidentiality reasons
Links to applicable Experimental skills
- Administration routes / Regimen
- Behavioural Science
- Confocal Microscopy
- Erection elicited by pharmacological or electrical neural stimulation
- Immunohistology
- Metabolic cages (diuresis, renal function, spontaneous micturition)
- Morphology
- Morphometry
- Non invasive blood pressure monitoring (tail cuff)
- Organ bath with animal tissues (In Vitro studies)
- Oxidative fluorescence
- Plasma / urine / tissue collection
- Protein expression and activity
- Spectrophotometric assays
- Telemetry
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